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破解說明:安裝完成後，請將光碟 \crack 目錄下的patcher，複製到主程式的
安裝目錄內中，並覆蓋!
"(install)\http\Dwww.premierbiosoft.com\P80\DMIUpdater\DMP2\DM210"
from cmd prompt run java -jar patcher.jar
軟體簡介:
Biosoft PrimerPlex v2.11.21103 PCR或測序引物以及雜交探針的設計評估軟體 英文破解版
官方網站：http://www.premierbiosoft.com/primerdesign/index.html
由加拿大的Premier公司開發的專業用於PCR或測序引物以及雜交探針的設計，評估的軟體，
其主要介面是分為序列編輯視窗（Genetank），引物設計視窗（Primer Design），瑂切分
析視窗（Restriction Sites）和紋基分析視窗（Motif）。
Primer Premier's search algorithm finds optimal PCR, multiplex, SNP genotyping
and degenerate primers with the most accurate melting temperature using the
nearest neighbor thermodynamic algorithm. Primers are screened for secondary
structures, dimers, hairpins, homologies and physical properties before reporting
the best ones for your sequence, in ranked order. Equipped with a handy calculator,
you can easily manipulate sequences and analyze the results of your primer design.
Load the gene of interest from NCBI, select a search range, sit back and let
Primer Premier pick the best possible primers for you.
Primer Design for SNP Genotyping Assays
With Primer Premier, you can load sequences from dbSNP and have the primers designed
flanking the SNP selected. Hundreds of unpublished SNPs can also be loaded by
specifying them as variation features in standard GenBank/dbSNP files. After specifying
the SNPs, primers can be designed to amplify them for detection using a probe-based
chemistry.
Multiplex Primer Design
For a multiplex experiment, Primer Premier enables you to design multiplex primers by
launching a primer search in batch mode and then checking the cross reactivity of the
primers designed. Primer Premier checks for all the possible reactivity and displays the
most stable structure formed by each oligo. This functionality reduces false priming and
ensures a strong signal strength.
Automatic Homology & Template Structure Avoidance
Primer Premier automatically interprets the BLAST search results and avoids those regions
to design primers that have significant cross homologies with the database. These homologous
regions are highlighted in the sequence view and are avoided during primer search.
Primer extension may be hindered due to the presence of template structures at extension
temperature. To avoid all such regions, where the template may fold upon itself, the program
utilizes a proprietary algorithm to check for possible secondary structures within the
template at a folding temperature you specify. The regions involved in the formation of a
secondary structure are underlined in the sequence view and are avoided while designing
primers.
Avoiding homologous regions makes the oligos highly specific and avoiding template structures
improves the efficiency of the designed primers.
